WEKO3
アイテム
{"_buckets": {"deposit": "521335fe-caae-473e-a43e-abb93749f3dc"}, "_deposit": {"created_by": 3, "id": "11462", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "11462"}, "status": "published"}, "_oai": {"id": "oai:ynu.repo.nii.ac.jp:00011462", "sets": ["496"]}, "author_link": ["40617", "37588", "37581", "40616", "40624", "37580", "40625", "40621", "40623", "40615", "40622"], "item_2_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-05-06", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "1001", "bibliographicPageStart": "992", "bibliographicVolumeNumber": "183", "bibliographic_titles": [{"bibliographic_title": "International Journal of Biological Macromolecules"}]}]}, "item_2_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge. Its sheath is assembled from a thiolic glycoconjugate called thiopeptidoglycan. S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans. However, it exhibits heat susceptibility, which distinguishes it from the S. natans sheath. In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation. The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence. The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans. The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC. Two derivatives were detected, and their structures were determined. We found that the sheath has no peptides and is assembled as follows: [→4)-β-D-GlcA-(1→4)-β-D-Glc-(1→3)-β-D-GalNAc-(1→4)-α-D-GalNAc-(1→4)-α-D-GalN-(1→]n (β-D-Glc and α-D-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively). Thiopeptidoglycan lyase was thus confirmed to cleave the 1,4 linkage between α-D-GalN and β-D-GlcA, regardless of the peptide moiety. Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath.", "subitem_description_type": "Abstract"}]}, "item_2_publisher_35": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_2_relation_13": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1016/j.ijbiomac.2021.05.001", "subitem_relation_type_select": "DOI"}}]}, "item_2_relation_44": {"attribute_name": "関係URI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://doi.org/10.1016/j.ijbiomac.2021.05.001"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.ijbiomac.2021.05.001", "subitem_relation_type_select": "DOI"}}]}, "item_2_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA1153092X", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "01418130", "subitem_source_identifier_type": "ISSN"}]}, "item_2_text_4": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "Faculty of Engineering, Yokohama National University"}, {"subitem_text_value": "Institute of Advanced Energy, Kyoto University"}, {"subitem_text_value": "Faculty of Engineering, Yokohama National University"}, {"subitem_text_value": "Faculty of Engineering, Yokohama National University"}, {"subitem_text_value": "Faculty of Engineering, Yokohama National University"}, {"subitem_text_value": "Faculty of Engineering, Yokohama National University"}, {"subitem_text_value": "School of Agriculture, Meiji University"}, {"subitem_text_value": "Faculty of Engineering, Yokohama National University"}, {"subitem_text_value": "Faculty of Engineering, Yokohama National University"}, {"subitem_text_value": "Institute of Advanced Energy, Kyoto University \u0026 Graduate School of Energy Science, Kyoto University"}, {"subitem_text_value": "Faculty of Engineering, Yokohama National University"}]}, "item_2_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kashiwabara, Daisuke"}], "nameIdentifiers": [{"nameIdentifier": "40621", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kondo Keiko"}], "nameIdentifiers": [{"nameIdentifier": "37581", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00707474", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00707474"}]}, {"creatorNames": [{"creatorName": "Usami Ryoji"}], "nameIdentifiers": [{"nameIdentifier": "40622", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kan Daisuke"}], "nameIdentifiers": [{"nameIdentifier": "40623", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawamura Izuru"}], "nameIdentifiers": [{"nameIdentifier": "40624", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kawasaki Yuta"}], "nameIdentifiers": [{"nameIdentifier": "40625", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato Michio"}], "nameIdentifiers": [{"nameIdentifier": "40615", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "00588775", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=00588775"}]}, {"creatorNames": [{"creatorName": "Nittami Tadashi"}], "nameIdentifiers": [{"nameIdentifier": "40616", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "20377089", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=20377089"}]}, {"creatorNames": [{"creatorName": "Suzuki Ichiro"}], "nameIdentifiers": [{"nameIdentifier": "40617", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "90303081", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=90303081"}]}, {"creatorNames": [{"creatorName": "Katahira Masato"}], "nameIdentifiers": [{"nameIdentifier": "37588", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "70211844", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70211844"}]}, {"creatorNames": [{"creatorName": "Takeda Minoru"}], "nameIdentifiers": [{"nameIdentifier": "37580", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "40247507", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40247507"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-12-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "J.Biol.Macromol.183.pp992-1001.pdf", "filesize": [{"value": "1.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1500000.0, "url": {"label": "J.Biol.Macromol.183.pp992-1001.pdf", "url": "https://ynu.repo.nii.ac.jp/record/11462/files/J.Biol.Macromol.183.pp992-1001.pdf"}, "version_id": "614cbf9e-199e-4a6d-91b0-428c8ebad2bc"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Sheath", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sphaerotilus montanus", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Thiopeptidoglycan lyase", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA"}]}, "item_type_id": "2", "owner": "3", "path": ["496"], "permalink_uri": "http://hdl.handle.net/10131/00014121", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-05-06"}, "publish_date": "2022-05-06", "publish_status": "0", "recid": "11462", "relation": {}, "relation_version_is_last": true, "title": ["Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA"], "weko_shared_id": -1}
Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA
http://hdl.handle.net/10131/00014121
http://hdl.handle.net/10131/000141211bbeb5a8-bd80-4a3b-b398-e1270a540e56
名前 / ファイル | ライセンス | アクション |
---|---|---|
J.Biol.Macromol.183.pp992-1001.pdf (1.5 MB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-05-06 | |||||
タイトル | ||||||
タイトル | Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Sheath, Sphaerotilus montanus, Thiopeptidoglycan lyase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kashiwabara, Daisuke
× Kashiwabara, Daisuke× Kondo Keiko× Usami Ryoji× Kan Daisuke× Kawamura Izuru× Kawasaki Yuta× Sato Michio× Nittami Tadashi× Suzuki Ichiro× Katahira Masato× Takeda Minoru |
|||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Institute of Advanced Energy, Kyoto University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
School of Agriculture, Meiji University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Institute of Advanced Energy, Kyoto University & Graduate School of Energy Science, Kyoto University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge. Its sheath is assembled from a thiolic glycoconjugate called thiopeptidoglycan. S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans. However, it exhibits heat susceptibility, which distinguishes it from the S. natans sheath. In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation. The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence. The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans. The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC. Two derivatives were detected, and their structures were determined. We found that the sheath has no peptides and is assembled as follows: [→4)-β-D-GlcA-(1→4)-β-D-Glc-(1→3)-β-D-GalNAc-(1→4)-α-D-GalNAc-(1→4)-α-D-GalN-(1→]n (β-D-Glc and α-D-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively). Thiopeptidoglycan lyase was thus confirmed to cleave the 1,4 linkage between α-D-GalN and β-D-GlcA, regardless of the peptide moiety. Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath. | |||||
書誌情報 |
International Journal of Biological Macromolecules 巻 183, p. 992-1001, 発行日 2021-05-06 |
|||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01418130 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1153092X | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.ijbiomac.2021.05.001 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
関係URI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.ijbiomac.2021.05.001 | |||||
関連名称 | https://doi.org/10.1016/j.ijbiomac.2021.05.001 |