{"created":"2023-06-20T15:14:20.601497+00:00","id":11462,"links":{},"metadata":{"_buckets":{"deposit":"521335fe-caae-473e-a43e-abb93749f3dc"},"_deposit":{"created_by":3,"id":"11462","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"11462"},"status":"published"},"_oai":{"id":"oai:ynu.repo.nii.ac.jp:00011462","sets":["495:496"]},"author_link":["40617","37588","37581","40616","40624","37580","40625","40621","40623","40615","40622"],"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2021-05-06","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"1001","bibliographicPageStart":"992","bibliographicVolumeNumber":"183","bibliographic_titles":[{"bibliographic_title":"International Journal of Biological Macromolecules"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Sphaerotilus natans is a filamentous sheath-forming bacterium commonly found in activated sludge. Its sheath is assembled from a thiolic glycoconjugate called thiopeptidoglycan. S. montanus ATCC-BAA-2725 is a sheath-forming member of stream biofilms, and its sheath is morphologically similar to that of S. natans. However, it exhibits heat susceptibility, which distinguishes it from the S. natans sheath. In this study, chemical composition and solid-state NMR analyses suggest that the S. montanus sheath is free of cysteine, indicating that disulfide linkage is not mandatory for sheath formation. The S. montanus sheath was successfully solubilized by N-acetylation, allowing solution-state NMR analysis to determine the sugar sequence. The sheath was susceptible to thiopeptidoglycan lyase prepared from the thiopeptidoglycan-assimilating bacterium, Paenibacillus koleovorans. The reducing ends of the enzymatic digests were labeled with 4-aminobenzoic acid ethyl ester, followed by HPLC. Two derivatives were detected, and their structures were determined. We found that the sheath has no peptides and is assembled as follows: [→4)-β-D-GlcA-(1→4)-β-D-Glc-(1→3)-β-D-GalNAc-(1→4)-α-D-GalNAc-(1→4)-α-D-GalN-(1→]n (β-D-Glc and α-D-GalNAc are stoichiometrically and substoichiometrically 3-O-acetylated, respectively). Thiopeptidoglycan lyase was thus confirmed to cleave the 1,4 linkage between α-D-GalN and β-D-GlcA, regardless of the peptide moiety. Furthermore, vital fluorescent staining of the sheath demonstrated that elongation takes place at the tips, as with the S. natans sheath.","subitem_description_type":"Abstract"}]},"item_2_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_2_relation_13":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.ijbiomac.2021.05.001","subitem_relation_type_select":"DOI"}}]},"item_2_relation_44":{"attribute_name":"関係URI","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.1016/j.ijbiomac.2021.05.001"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.ijbiomac.2021.05.001","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA1153092X","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01418130","subitem_source_identifier_type":"ISSN"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Institute of Advanced Energy, Kyoto University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"School of Agriculture, Meiji University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Institute of Advanced Energy, Kyoto University & Graduate School of Energy Science, Kyoto University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kashiwabara, Daisuke"}],"nameIdentifiers":[{"nameIdentifier":"40621","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kondo  Keiko"}],"nameIdentifiers":[{"nameIdentifier":"37581","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00707474","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00707474"}]},{"creatorNames":[{"creatorName":"Usami  Ryoji"}],"nameIdentifiers":[{"nameIdentifier":"40622","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kan  Daisuke"}],"nameIdentifiers":[{"nameIdentifier":"40623","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawamura  Izuru"}],"nameIdentifiers":[{"nameIdentifier":"40624","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawasaki  Yuta"}],"nameIdentifiers":[{"nameIdentifier":"40625","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato  Michio"}],"nameIdentifiers":[{"nameIdentifier":"40615","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00588775","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00588775"}]},{"creatorNames":[{"creatorName":"Nittami  Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"40616","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20377089","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20377089"}]},{"creatorNames":[{"creatorName":"Suzuki  Ichiro"}],"nameIdentifiers":[{"nameIdentifier":"40617","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90303081","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90303081"}]},{"creatorNames":[{"creatorName":"Katahira  Masato"}],"nameIdentifiers":[{"nameIdentifier":"37588","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70211844","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70211844"}]},{"creatorNames":[{"creatorName":"Takeda  Minoru"}],"nameIdentifiers":[{"nameIdentifier":"37580","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40247507","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40247507"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-12-15"}],"displaytype":"detail","filename":"J.Biol.Macromol.183.pp992-1001.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"J.Biol.Macromol.183.pp992-1001.pdf","url":"https://ynu.repo.nii.ac.jp/record/11462/files/J.Biol.Macromol.183.pp992-1001.pdf"},"version_id":"614cbf9e-199e-4a6d-91b0-428c8ebad2bc"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Sheath","subitem_subject_scheme":"Other"},{"subitem_subject":"Sphaerotilus montanus","subitem_subject_scheme":"Other"},{"subitem_subject":"Thiopeptidoglycan lyase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA"}]},"item_type_id":"2","owner":"3","path":["496"],"pubdate":{"attribute_name":"公開日","attribute_value":"2022-05-06"},"publish_date":"2022-05-06","publish_status":"0","recid":"11462","relation_version_is_last":true,"title":["Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-D-GalN and β-D-GlcA"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T17:55:00.829351+00:00"}