{"created":"2023-06-20T15:13:01.312483+00:00","id":9968,"links":{},"metadata":{"_buckets":{"deposit":"6bcb3fc1-80a8-4e89-ba25-45b6479230a3"},"_deposit":{"created_by":3,"id":"9968","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9968"},"status":"published"},"_oai":{"id":"oai:ynu.repo.nii.ac.jp:00009968","sets":["495:496"]},"author_link":["35847","35841","35840","35845","35846","35842","35844","35843"],"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-10-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"167","bibliographicPageStart":"161","bibliographicVolumeNumber":"117","bibliographic_titles":[{"bibliographic_title":"Biosensors and Bioelectronics"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The emission intensity of GaInAsP semiconductors that show an ion sensitivity is altered by the surface charge. In this study, we propose a biosensing technique using GaInAsP photonic crystal nanolasers based on this principle. Here, simple and rapid detection of collapsin response mediator protein 2 (CRMP2) is demonstrated, which is a promising biomarker candidate for neuropsychiatric diseases existing in peripheral white blood cells. We prepared CRMP2 as a standard protein and introduced sodium dodecyl sulfate (SDS) as an anionic surfactant to enhance the net negative charge of the protein. The nanolaser was modified in advance with an anti-CRMP2 antibody and then photopumped at a constant power. The laser emission intensity was monitored during the antibody–antigen reaction. Consequently, CRMP2 was detected as a decrease in the emission intensity. We achieved a lower limit for detection of 3.8 μg/mL that satisfies the requirement for clinical biomarker testing. Without the requirements of any kind of labels and spectral analyses, this technique allows for simple, rapid, and low-cost biomarker detection.","subitem_description_type":"Abstract"}]},"item_2_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_2_relation_13":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bios.2018.05.059","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09565663","subitem_source_identifier_type":"ISSN"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Department of Electrical and Computer Engineering, Yokohama National University"},{"subitem_text_value":"Department of Molecular Pharmacology and Neurobiology, Yokohama City University"},{"subitem_text_value":"Department of Molecular Pharmacology and Neurobiology, Yokohama City University"},{"subitem_text_value":"Department of Electrical and Computer Engineering, Yokohama National University"},{"subitem_text_value":"Department of Electrical and Computer Engineering, Yokohama National University"},{"subitem_text_value":"Department of Electrical and Computer Engineering, Yokohama National University"},{"subitem_text_value":"Department of Molecular Pharmacology and Neurobiology, Yokohama City University"},{"subitem_text_value":"Department of Electrical and Computer Engineering, Yokohama National University"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Watanabe, Keisuke"}],"nameIdentifiers":[{"nameIdentifier":"35841","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nomoto, Munetaka"}],"nameIdentifiers":[{"nameIdentifier":"35842","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80457861","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80457861"}]},{"creatorNames":[{"creatorName":"Nakamura, Fumio"}],"nameIdentifiers":[{"nameIdentifier":"35843","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hachuda, Shoji"}],"nameIdentifiers":[{"nameIdentifier":"35844","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakata, Akihiro"}],"nameIdentifiers":[{"nameIdentifier":"35845","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Takumi"}],"nameIdentifiers":[{"nameIdentifier":"35846","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Goshima, Yoshio"}],"nameIdentifiers":[{"nameIdentifier":"35847","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00153750","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00153750"}]},{"creatorNames":[{"creatorName":"Baba, Toshihiko"}],"nameIdentifiers":[{"nameIdentifier":"35840","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50202271","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50202271"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-10-15"}],"displaytype":"detail","filename":"Biosens_and_bioele_watanabe_manuscript_final_rev3.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Biosens_and_bioele_watanabe_manuscript_final_rev3.pdf","url":"https://ynu.repo.nii.ac.jp/record/9968/files/Biosens_and_bioele_watanabe_manuscript_final_rev3.pdf"},"version_id":"4c2fce0b-0a54-4667-ac12-77a0e1042526"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"GaInAsP","subitem_subject_scheme":"Other"},{"subitem_subject":"Label-free","subitem_subject_scheme":"Other"},{"subitem_subject":"Photonic crystal","subitem_subject_scheme":"Other"},{"subitem_subject":"Nanolaser","subitem_subject_scheme":"Other"},{"subitem_subject":"Biomarker","subitem_subject_scheme":"Other"},{"subitem_subject":"Collapsin response mediator protein","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Label-free and spectral-analysis-free detection of neuropsychiatric disease biomarkers using an ion-sensitive GaInAsP nanolaser biosensor","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Label-free and spectral-analysis-free detection of neuropsychiatric disease biomarkers using an ion-sensitive GaInAsP nanolaser biosensor"}]},"item_type_id":"2","owner":"3","path":["496"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-06-13"},"publish_date":"2019-06-13","publish_status":"0","recid":"9968","relation_version_is_last":true,"title":["Label-free and spectral-analysis-free detection of neuropsychiatric disease biomarkers using an ion-sensitive GaInAsP nanolaser biosensor"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T19:00:41.393727+00:00"}