@article{oai:ynu.repo.nii.ac.jp:00009968,
 author = {Watanabe, Keisuke and Nomoto, Munetaka and Nakamura, Fumio and Hachuda, Shoji and Sakata, Akihiro and Watanabe, Takumi and Goshima, Yoshio and Baba, Toshihiko},
 journal = {Biosensors and Bioelectronics},
 month = {Oct},
 note = {The emission intensity of GaInAsP semiconductors that show an ion sensitivity is altered by the surface charge. In this study, we propose a biosensing technique using GaInAsP photonic crystal nanolasers based on this principle. Here, simple and rapid detection of collapsin response mediator protein 2 (CRMP2) is demonstrated, which is a promising biomarker candidate for neuropsychiatric diseases existing in peripheral white blood cells. We prepared CRMP2 as a standard protein and introduced sodium dodecyl sulfate (SDS) as an anionic surfactant to enhance the net negative charge of the protein. The nanolaser was modified in advance with an anti-CRMP2 antibody and then photopumped at a constant power. The laser emission intensity was monitored during the antibody–antigen reaction. Consequently, CRMP2 was detected as a decrease in the emission intensity. We achieved a lower limit for detection of 3.8 μg/mL that satisfies the requirement for clinical biomarker testing. Without the requirements of any kind of labels and spectral analyses, this technique allows for simple, rapid, and low-cost biomarker detection.},
 pages = {161--167},
 title = {Label-free and spectral-analysis-free detection of neuropsychiatric disease biomarkers using an ion-sensitive GaInAsP nanolaser biosensor},
 volume = {117},
 year = {2018}
}