{"created":"2023-06-20T15:12:42.158676+00:00","id":9529,"links":{},"metadata":{"_buckets":{"deposit":"6d014d71-4c75-4161-a7d8-4e10ee5e954c"},"_deposit":{"created_by":3,"id":"9529","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"9529"},"status":"published"},"_oai":{"id":"oai:ynu.repo.nii.ac.jp:00009529","sets":["495:496"]},"author_link":["35033","35029","35034","34923","35031","35032","35035","35030"],"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-07-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"83","bibliographicPageStart":"72","bibliographicVolumeNumber":"115","bibliographic_titles":[{"bibliographic_title":"Biophysical Journal"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pharanois phoborhodopsin (ppR) from Natronomonas pharaonis is a transmembrane photoreceptor protein involved in negative phototaxis. Structural changes in ppR triggered by photoisomerization of the retinal chromophore are transmitted to its cognate transducer protein (pHtrII) through a cyclic photoreaction pathway involving several photointermediates. This pathway is called the photocycle. It is important to understand the detailed configurational changes of retinal during the photocycle. We previously observed one of the photointermediates (M-intermediates) by in situ photoirradiation solid-state NMR experiments. In this study, we further observed the 13C cross-polarization magic-angle-spinning NMR signals of late photointermediates such as O- and N′-intermediates by illumination with green light (520 nm). Under blue-light (365 nm) irradiation of the M-intermediates, 13C cross-polarization magic-angle-spinning NMR signals of 14- and 20-13C-labeled retinal in the O-intermediate appeared at 115.4 and 16.4 ppm and were assigned to the 13-trans, 15-syn configuration. The signals caused by the N′-intermediate appeared at 115.4 and 23.9 ppm and were assigned to the 13-cis configuration, and they were in an equilibrium state with the O-intermediate during thermal decay of the M-intermediates at −60°C. Thus, photoirradiation NMR studies revealed the photoreaction pathways from the M- to O-intermediates and the equilibrium state between the N′- and O-intermediate. Further, we evaluated the detailed retinal configurations in the O- and N′-intermediates by performing a density functional theory chemical shift calculation. The results showed that the N′-intermediate has a 63° twisted retinal state due to the 13-cis configuration. The retinal configurations of the O- and N′-intermediates were determined to be 13-trans, 15-syn, and 13-cis, respectively, based on the chemical shift values of [20-13C] and [14-13C] retinal obtained by photoirradiation solid-state NMR and density functional theory calculation.","subitem_description_type":"Abstract"}]},"item_2_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B.V.  "}]},"item_2_relation_13":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.bpj.2018.05.030","subitem_relation_type_select":"DOI"}}]},"item_2_rights_14":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"©2018, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00063495","subitem_source_identifier_type":"ISSN"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Engineering, Yokohama National University"},{"subitem_text_value":"Graduate School of Engineering, Yokohama National University"},{"subitem_text_value":"Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University"},{"subitem_text_value":"Laboratory of Organic Chemistry for Life Science, Kobe Pharmaceutical University"},{"subitem_text_value":"Faculty of Advanced Life Science, Hokkaido University"},{"subitem_text_value":"Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University"},{"subitem_text_value":"Graduate School of Engineering, Yokohama National University"},{"subitem_text_value":"Graduate School of Engineering, Yokohama National University"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Makino, Yoshiteru"}],"nameIdentifiers":[{"nameIdentifier":"35029","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"kawamura, izuru"}],"nameIdentifiers":[{"nameIdentifier":"34923","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20452047","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20452047"}]},{"creatorNames":[{"creatorName":"Okitsu, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"35035","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wada, Akimori"}],"nameIdentifiers":[{"nameIdentifier":"35030","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamo, Naoki"}],"nameIdentifiers":[{"nameIdentifier":"35031","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sudo, Yuki"}],"nameIdentifiers":[{"nameIdentifier":"35032","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ueda, Kazuyoshi"}],"nameIdentifiers":[{"nameIdentifier":"35033","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Naito, Akira "}],"nameIdentifiers":[{"nameIdentifier":"35034","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-04"}],"displaytype":"detail","filename":"29827_1_merged_1527771477.pdf","filesize":[{"value":"21.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"29827_1_merged_1527771477","url":"https://ynu.repo.nii.ac.jp/record/9529/files/29827_1_merged_1527771477.pdf"},"version_id":"79b3426f-f75f-44e9-8bd5-119da144cf10"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Retinal Configuration of ppR Intermediates Revealed by Photoirradiation Solid-State NMR and DFT","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Retinal Configuration of ppR Intermediates Revealed by Photoirradiation Solid-State NMR and DFT"}]},"item_type_id":"2","owner":"3","path":["496"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-02-12"},"publish_date":"2019-02-12","publish_status":"0","recid":"9529","relation_version_is_last":true,"title":["Retinal Configuration of ppR Intermediates Revealed by Photoirradiation Solid-State NMR and DFT"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T18:22:36.975906+00:00"}