{"created":"2023-06-20T15:08:54.772619+00:00","id":4769,"links":{},"metadata":{"_buckets":{"deposit":"68867cb2-d97e-40d6-900c-0fee312d7f49"},"_deposit":{"created_by":3,"id":"4769","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4769"},"status":"published"},"_oai":{"id":"oai:ynu.repo.nii.ac.jp:00004769","sets":["500:501"]},"author_link":["22078","22075","22077","22076","22079"],"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"45","bibliographicPageStart":"40","bibliographicVolumeNumber":"223","bibliographic_titles":[{"bibliographic_title":"Planta"}]}]},"item_2_description_17":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"},{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_description_42":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"postprint","subitem_description_type":"Other"}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Efficiency of novel fiber formation was much improved in protoplast culture of embryogenic cells (ECs) of a conifer, Larix leptolepis (Sieb. et Zucc.) Gord., by pre-culturing ECs in a medium containing a high concentration of glutamine (13.7 mM). The fibrillar substructures of large and elongated fibers of protoplasts isolated from Larix ECs were investigated by laser confocal scanning microscopy (LCSM) after Aniline Blue staining and atomic force microscopy (AFM) using a micromanipulator without any pre-treatment. Fibers were composed of bundles of fibrils and subfibrils, whose diameters were defined as 0.7 and 0.17 mu m, respectively, by image analysis after LCSM and AFM. These fibers were proven to be composed of callose by using specific degrading enzymes for beta-1,4-glucan and beta-1,3-glucan.","subitem_description_type":"Abstract"}]},"item_2_full_name_2":{"attribute_name":"著者(ヨミ)","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{"name":"ササモト, ハマコ"}]}]},"item_2_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{}],"names":[{}]}]},"item_2_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer"}]},"item_2_relation_13":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1007/s00425-005-0065-3","subitem_relation_type_select":"DOI"}}]},"item_2_rights_14":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The original publication is available at www.springerlink.com."}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00775481","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00320935","subitem_source_identifier_type":"ISSN"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Yokohama Natl Univ, Fac Environm & Informat Sci, Kanagawa 2408501, Japan"},{"subitem_text_value":"Forestry & Forest Prod Res Inst, Tsukuba, Ibaraki 3058687, Japan"},{"subitem_text_value":"横浜国立大学　環境情報研究院　自然環境と情報部門　環境遺伝子工学分野"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fukumoto, T."}],"nameIdentifiers":[{"nameIdentifier":"22075","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hayashi, N."}],"nameIdentifiers":[{"nameIdentifier":"22076","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sasamoto, Hamako"}],"nameIdentifiers":[{"nameIdentifier":"22077","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-09-16"}],"displaytype":"detail","filename":"ISI-000233725000005-01.pdf","filesize":[{"value":"154.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISI-000233725000005-01.pdf","url":"https://ynu.repo.nii.ac.jp/record/4769/files/ISI-000233725000005-01.pdf"},"version_id":"38bbcf2b-87b8-4cee-9c03-b35a2b3a7a1f"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-09-16"}],"displaytype":"detail","filename":"ISI-000233725000005-02.pdf","filesize":[{"value":"443.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ISI-000233725000005-02.pdf","url":"https://ynu.repo.nii.ac.jp/record/4769/files/ISI-000233725000005-02.pdf"},"version_id":"86f5614b-f39a-427b-afeb-03530dcb26c3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AFM","subitem_subject_scheme":"Other"},{"subitem_subject":"beta-1,3-glucan","subitem_subject_scheme":"Other"},{"subitem_subject":"embryogenic cells","subitem_subject_scheme":"Other"},{"subitem_subject":"larix","subitem_subject_scheme":"Other"},{"subitem_subject":"LCSM","subitem_subject_scheme":"Other"},{"subitem_subject":"protoplast fiber","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Atomic force microscopy and laser confocal scanning microscopy analysis of callose fibers developed from protoplasts of embryogenic cells of a conifer","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Atomic force microscopy and laser confocal scanning microscopy analysis of callose fibers developed from protoplasts of embryogenic cells of a conifer"}]},"item_type_id":"2","owner":"3","path":["501"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-06-07"},"publish_date":"2007-06-07","publish_status":"0","recid":"4769","relation_version_is_last":true,"title":["Atomic force microscopy and laser confocal scanning microscopy analysis of callose fibers developed from protoplasts of embryogenic cells of a conifer"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T19:39:11.962508+00:00"}