| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-09-25 |
| タイトル |
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|
タイトル |
Effects of Exosomes Derived from Dermal Papilla Cells on Hair Follicle Stem Cells and Hair Follicle Organoids |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
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|
主題 |
dermal papilla cells, hair follicle stem cells, organoid, exosome, hair growth(en) |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Zhou, Yinghui
Yamane, Monami
Suzuki, Kohei
| en |
Suzuki, Kohei
Yokohama National University
Nissan Chemical Corporation
|
Search repository
Nanmo, Ayaka
Tu, Shan
Kageyama, Tatsuto
Fukuda, Junji
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Hair follicle development and cycle are regulated by the reciprocal interactions between epithelial and mesenchymal cells. Mechanisms responsible for the regulation have mainly been studied using experimental animals such as rodent fetuses. The purpose of this study is to understand such communications by using in vitro cell culture models of human hair follicle-derived cells. Human dermal papilla cells (DPCs) were cultured using 2-dimensional (2D), spheroid (3D), and spheroid microwell array cultures in an oxygen-permeable substrate (3D-oxy). Exosomes secreted in the culture media were collected and purified. All three types of exosomes moderately promoted proliferation of human hair follicle stem cells (HFSCs). Exosomes from the 3D-oxy culture most efficiently upregulated the expression of CD200, CD34, and K15 in HFSCs compared to those from the other two culture methods. To investigate the effects of exosomes on in vitro hair follicle formation, mouse embryonic epithelial and mesenchymal cells were used to prepare hair follicle organoids. The expression analysis of trichogenic and HFSC marker genes revealed that exosomes from the 3D-oxy culture were the most effective in maintaining stem cells and stimulating hair follicle sprouting in vitro. Exosomes from human DPCs may have substantial potential for treating patients with hair loss. |
|
言語 |
en |
| 書誌情報 |
en : Alternatives to Animal Testing and Experimentation
巻 27,
号 1,
p. 1-13,
ページ数 13,
発行日 2022
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| ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
13440411 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11161787 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.11232/aatex.27.1 |
| 権利 |
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|
権利情報Resource |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
|
言語 |
en |
| 著者版フラグ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
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|
出版者 |
日本動物実験代替法学会 |
27_aatex27_1.pdf (1.2 MB)
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