| 著者 |
Sugiyama, Ellen
Nanmo, Ayaka
Nie, Xiaolei
ORCID
0000-0003-4946-1978
| en |
Nie, Xiaolei
Fac Engn, Pillar Engn Prod Dev, Singapore University of Technology & Design
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Singapore University of Technology & Design
|
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Chang, Shu-Yung
| en |
Chang, Shu-Yung
Fac Engn, Pillar Engn Prod Dev, Singapore University of Technology & Design
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Singapore University of Technology & Design
|
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Hashimoto, Michinao
| en |
Hashimoto, Michinao
Fac Engn, Pillar Engn Prod Dev, Singapore University of Technology & Design
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Singapore University of Technology & Design
|
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Suzuki, Atsushi
| en |
Suzuki, Atsushi
Fac Engn, Yokohama National University
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Inst Adv Sci, Yokohama National University
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Kageyama, Tatsuto
ORCID
0000-0001-6248-3755
| en |
Kageyama, Tatsuto
Fac Engn, Yokohama National University
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Kanagawa Academy Science & Technology
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Inst Adv Sci, Yokohama National University
|
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Fukuda, Junji
ORCID
0000-0002-7194-8099
e-Rad_Researcher
80431675
| en |
Fukuda, Junji
Fac Engn, Yokohama National University
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Kanagawa Academy Science & Technology
Fac Engn, Pillar Engn Prod Dev, Digital Mfg & Design DManD Ctr, Inst Adv Sci, Yokohama National University
|
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内容記述 |
Hair follicle morphogenesis during embryonic development is driven by the formation of hair follicle germs (HFGs) via interactions between epithelial and mesenchymal cells. Bioengineered HFGs are potential tissue grafts for hair regenerative medicine because they can replicate interactions and hair follicle morphogenesis after transplantation. However, a mass preparation approach for HFGs is necessary for clinical applications, given that thousands of de novo hair follicles are required to improve the appearance of a single patient with alopecia. In this study, we developed a microfluidics-based approach for the large-scale preparation of HFGs. A simple flow-focusing microfluidic device allowed collagen solutions containing epithelial and mesenchymal cells to flow and generate collagen microbeads with distinct Janus structures. During the 3 days of culture, the collagen beads contracted owing to cellular traction forces, resulting in collagen- and cell-dense HFGs. The transplantation of HFGs into nude mice resulted in highly efficient de novo hair follicle regeneration. This method provides a scalable and robust tissue graft preparation approach for hair regeneration. |
sugiyama-et-al-2024-large-scale-preparation-of-hair-follicle-germs-using-a-microfluidic-device.pdf (4.6 MB)
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