{"created":"2023-06-20T15:13:05.687462+00:00","id":10049,"links":{},"metadata":{"_buckets":{"deposit":"6342a17d-8e2b-4e59-847f-b3ae38222f9c"},"_deposit":{"created_by":3,"id":"10049","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10049"},"status":"published"},"_oai":{"id":"oai:ynu.repo.nii.ac.jp:00010049","sets":["495:496"]},"author_link":["34841","36010","36011","36012","36009"],"item_2_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"63","bibliographicPageStart":"55","bibliographicVolumeNumber":"212","bibliographic_titles":[{"bibliographic_title":"Biomaterials"}]}]},"item_2_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Hair regenerative medicine is a promising approach for hair loss, during which autologous follicular stem cells are transplanted into regions of hair loss to regenerate hairs. Because cells transplanted as a single cell suspension scarcely generate hairs, the engineering of three-dimensional (3D) tissues before transplantation has been explored to improve this process. Here, we propose an approach to fabricate collagen-enriched cell aggregates, named hair beads (HBs), through the spontaneous constriction of cell-encapsulated collagen drops. Mouse embryonic mesenchymal cells or human dermal papilla cells were encapsulated in 2-μl collagen microgels, which were concentrated >10-fold in volume during 3 days of culture. Interestingly, HB constriction was attributed to attraction forces driven by myosin II and involved the upregulation of follicular genes. Single HBs with epithelial cells seeded in U-shaped microwells formed dumbbell-like structures comprising respective aggregates (named bead-based hair follicle germs, bbHFGs), during 3 days of culture. bbHFGs efficiently generated hair follicles upon intracutaneous transplantation into the backs of nude mice. Using an automated spotter, this approach was scalable to prepare a large number of bbHFGs, which is important for clinical applications. Therefore, this could represent a robust and practical approach for the preparation of germ-like tissues for hair regenerative ","subitem_description_type":"Abstract"}]},"item_2_publisher_35":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_2_relation_13":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1016/j.biomaterials.2019.05.003","subitem_relation_type_select":"DOI"}}]},"item_2_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00110092","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01429612","subitem_source_identifier_type":"ISSN"}]},"item_2_text_4":{"attribute_name":"著者所属","attribute_value_mlt":[{"subitem_text_value":"Faculty of Engineering, Yokohama National University / Kanagawa Institute of Industrial Science and Technology"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University"},{"subitem_text_value":"Faculty of Engineering, Yokohama National University / Kanagawa Institute of Industrial Science and Technology"}]},"item_2_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kageyama, Tatsuto"}],"nameIdentifiers":[{"nameIdentifier":"36009","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yan, Lei"}],"nameIdentifiers":[{"nameIdentifier":"36010","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimizu, Akihiro"}],"nameIdentifiers":[{"nameIdentifier":"36011","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maruo, Shoji"}],"nameIdentifiers":[{"nameIdentifier":"36012","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00314047","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00314047"}]},{"creatorNames":[{"creatorName":"Fukuda, Junji"}],"nameIdentifiers":[{"nameIdentifier":"34841","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80431675","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80431675"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-22"}],"displaytype":"detail","filename":"1-s2.0-S0142961219302674-main.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1-s2.0-S0142961219302674-main.pdf","url":"https://ynu.repo.nii.ac.jp/record/10049/files/1-s2.0-S0142961219302674-main.pdf"},"version_id":"37ba1302-3cff-42c6-ba54-0be8ac85473e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Hair regenerative medicine","subitem_subject_scheme":"Other"},{"subitem_subject":"Dermal papilla cell","subitem_subject_scheme":"Other"},{"subitem_subject":"Collagen microgel","subitem_subject_scheme":"Other"},{"subitem_subject":"Hair follicle germ","subitem_subject_scheme":"Other"},{"subitem_subject":"Attraction force","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Preparation of hair beads and hair follicle germs for regenerative medicine","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Preparation of hair beads and hair follicle germs for regenerative medicine"}]},"item_type_id":"2","owner":"3","path":["496"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-07-22"},"publish_date":"2019-07-22","publish_status":"0","recid":"10049","relation_version_is_last":true,"title":["Preparation of hair beads and hair follicle germs for regenerative medicine"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T18:48:34.049817+00:00"}