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Atomic force microscopy and laser confocal scanning microscopy analysis of callose fibers developed from protoplasts of embryogenic cells of a conifer
http://hdl.handle.net/10131/932
http://hdl.handle.net/10131/932fcc33a50-5e88-4f31-9dca-eccf504204d1
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ISI-000233725000005-01.pdf (154.6 kB)
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ISI-000233725000005-02.pdf (443.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-06-07 | |||||
タイトル | ||||||
タイトル | Atomic force microscopy and laser confocal scanning microscopy analysis of callose fibers developed from protoplasts of embryogenic cells of a conifer | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | AFM, beta-1,3-glucan, embryogenic cells, larix, LCSM, protoplast fiber | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Fukumoto, T.
× Fukumoto, T.× Hayashi, N.× Sasamoto, Hamako |
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著者(ヨミ) | ||||||
識別子Scheme | WEKO | |||||
識別子 | 22078 | |||||
姓名 | ササモト, ハマコ | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 22079 | |||||
姓名 | 笹本, 浜子 | |||||
著者所属 | ||||||
Yokohama Natl Univ, Fac Environm & Informat Sci, Kanagawa 2408501, Japan | ||||||
著者所属 | ||||||
Forestry & Forest Prod Res Inst, Tsukuba, Ibaraki 3058687, Japan | ||||||
著者所属 | ||||||
横浜国立大学 環境情報研究院 自然環境と情報部門 環境遺伝子工学分野 | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Efficiency of novel fiber formation was much improved in protoplast culture of embryogenic cells (ECs) of a conifer, Larix leptolepis (Sieb. et Zucc.) Gord., by pre-culturing ECs in a medium containing a high concentration of glutamine (13.7 mM). The fibrillar substructures of large and elongated fibers of protoplasts isolated from Larix ECs were investigated by laser confocal scanning microscopy (LCSM) after Aniline Blue staining and atomic force microscopy (AFM) using a micromanipulator without any pre-treatment. Fibers were composed of bundles of fibrils and subfibrils, whose diameters were defined as 0.7 and 0.17 mu m, respectively, by image analysis after LCSM and AFM. These fibers were proven to be composed of callose by using specific degrading enzymes for beta-1,4-glucan and beta-1,3-glucan. | |||||
書誌情報 |
Planta 巻 223, 号 1, p. 40-45, 発行日 2005-12 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00320935 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00775481 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1007/s00425-005-0065-3 | |||||
権利 | ||||||
権利情報 | The original publication is available at www.springerlink.com. | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
出版者 | ||||||
出版者 | Springer | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | postprint |