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Preparation of hair beads and hair follicle germs for regenerative medicine
http://hdl.handle.net/10131/00012714
http://hdl.handle.net/10131/0001271413ed323a-31a7-4325-b006-5460cef66a05
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S0142961219302674-main.pdf (2.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-07-22 | |||||
タイトル | ||||||
タイトル | Preparation of hair beads and hair follicle germs for regenerative medicine | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Hair regenerative medicine, Dermal papilla cell, Collagen microgel, Hair follicle germ, Attraction force | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kageyama, Tatsuto
× Kageyama, Tatsuto× Yan, Lei× Shimizu, Akihiro× Maruo, Shoji× Fukuda, Junji |
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著者所属 | ||||||
Faculty of Engineering, Yokohama National University / Kanagawa Institute of Industrial Science and Technology | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Faculty of Engineering, Yokohama National University / Kanagawa Institute of Industrial Science and Technology | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Hair regenerative medicine is a promising approach for hair loss, during which autologous follicular stem cells are transplanted into regions of hair loss to regenerate hairs. Because cells transplanted as a single cell suspension scarcely generate hairs, the engineering of three-dimensional (3D) tissues before transplantation has been explored to improve this process. Here, we propose an approach to fabricate collagen-enriched cell aggregates, named hair beads (HBs), through the spontaneous constriction of cell-encapsulated collagen drops. Mouse embryonic mesenchymal cells or human dermal papilla cells were encapsulated in 2-μl collagen microgels, which were concentrated >10-fold in volume during 3 days of culture. Interestingly, HB constriction was attributed to attraction forces driven by myosin II and involved the upregulation of follicular genes. Single HBs with epithelial cells seeded in U-shaped microwells formed dumbbell-like structures comprising respective aggregates (named bead-based hair follicle germs, bbHFGs), during 3 days of culture. bbHFGs efficiently generated hair follicles upon intracutaneous transplantation into the backs of nude mice. Using an automated spotter, this approach was scalable to prepare a large number of bbHFGs, which is important for clinical applications. Therefore, this could represent a robust and practical approach for the preparation of germ-like tissues for hair regenerative | |||||
書誌情報 |
Biomaterials 巻 212, p. 55-63, 発行日 2019-08 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01429612 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00110092 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.biomaterials.2019.05.003 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | Elsevier |