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Label-free and spectral-analysis-free detection of neuropsychiatric disease biomarkers using an ion-sensitive GaInAsP nanolaser biosensor
http://hdl.handle.net/10131/00012634
http://hdl.handle.net/10131/000126342e1a7157-d863-4799-ad5c-3e1337f885c4
名前 / ファイル | ライセンス | アクション |
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Biosens_and_bioele_watanabe_manuscript_final_rev3.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-06-13 | |||||
タイトル | ||||||
タイトル | Label-free and spectral-analysis-free detection of neuropsychiatric disease biomarkers using an ion-sensitive GaInAsP nanolaser biosensor | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | GaInAsP, Label-free, Photonic crystal, Nanolaser, Biomarker, Collapsin response mediator protein | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Watanabe, Keisuke
× Watanabe, Keisuke× Nomoto, Munetaka× Nakamura, Fumio× Hachuda, Shoji× Sakata, Akihiro× Watanabe, Takumi× Goshima, Yoshio× Baba, Toshihiko |
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著者所属 | ||||||
Department of Electrical and Computer Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Department of Molecular Pharmacology and Neurobiology, Yokohama City University | ||||||
著者所属 | ||||||
Department of Molecular Pharmacology and Neurobiology, Yokohama City University | ||||||
著者所属 | ||||||
Department of Electrical and Computer Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Department of Electrical and Computer Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Department of Electrical and Computer Engineering, Yokohama National University | ||||||
著者所属 | ||||||
Department of Molecular Pharmacology and Neurobiology, Yokohama City University | ||||||
著者所属 | ||||||
Department of Electrical and Computer Engineering, Yokohama National University | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The emission intensity of GaInAsP semiconductors that show an ion sensitivity is altered by the surface charge. In this study, we propose a biosensing technique using GaInAsP photonic crystal nanolasers based on this principle. Here, simple and rapid detection of collapsin response mediator protein 2 (CRMP2) is demonstrated, which is a promising biomarker candidate for neuropsychiatric diseases existing in peripheral white blood cells. We prepared CRMP2 as a standard protein and introduced sodium dodecyl sulfate (SDS) as an anionic surfactant to enhance the net negative charge of the protein. The nanolaser was modified in advance with an anti-CRMP2 antibody and then photopumped at a constant power. The laser emission intensity was monitored during the antibody–antigen reaction. Consequently, CRMP2 was detected as a decrease in the emission intensity. We achieved a lower limit for detection of 3.8 μg/mL that satisfies the requirement for clinical biomarker testing. Without the requirements of any kind of labels and spectral analyses, this technique allows for simple, rapid, and low-cost biomarker detection. | |||||
書誌情報 |
Biosensors and Bioelectronics 巻 117, p. 161-167, 発行日 2018-10-15 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09565663 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.bios.2018.05.059 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
出版者 | ||||||
出版者 | Elsevier |